Figure 3:

Growth curves of P. gingivalis W83 showing the antibacterial activities of aerobically prepared nanoparticles. Bacterial growth over a 24-hour period was assessed by measuring the absorbance of cultures at 600 nm for specific intervals. P. gingivalis W83 was inoculated with aerobically prepared Ag nanoparticles coated with F127 (≈0.3 nM, 500 μL) or glutathione capped (≈0.3 nM, 500 μL) and Ag/Au (Au: Ag = 0.30 ± 0.05; ≈0.3 nM, 500 μL) nanoparticles in 5 mL total volume. Glutathione capping was done using method II. Error bars represent the standard deviation of three experiments.