Figure 4:

Growth curves of P. gingivalis W83 comparing the antibacterial activity of aerobically prepared nanoparticles with 0.5 mM AgNO₃. Bacterial growth over a 24-hour period was assessed by measuring the absorbance of cultures at 600 nm for specific intervals. P. gingivalis W83 (OD_600nm ≈ 0.15) was challenged with water (400 μL), maltose coated Ag (≈0.2 nM, 400 μL; OD_λmax = 1) nanoparticles, or 0.5 mM AgNO (6.25 mM, 400 μL) in 5 mL total volume. Glutathione capping was done using method I. Error bars represent the standard deviation of three experiments.