TABLE 2.
Strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| Enterobacter soli LF7 | Can utilize IAA as sole carbon source (this study) | 8 |
| Enterobacter soli LF7 ΔiacA::cat | E. soli LF7 derivative; iacA replaced with cat gene | This study |
| Enterobacter soli LF7 ΔiacR::cat | E. soli LF7 derivative; iacR replaced with cat gene | This study |
| Escherichia coli TOP10 | Cloning host | Thermo Fisher Scientific |
| Escherichia coli MB613 | Host of pKD3 harboring cat gene | 32 |
| Escherichia coli MB616 | Host of pKD46 | 32 |
| Plasmids | ||
| pPROBE′-gfp[tagless] | gfp expression vector | 10 |
| pPROBE′-gfp[LVA] | gfp[LVA] expression vector | 10 |
| pPROBE′-gfp[AAV] | gfp[AAV] expression vector | 10 |
| pPROBE′-gfp[ASV] | gfp[ASV] expression vector | 10 |
| pCR2.1TOPO | PCR amplicon cloning vector | Thermo Fisher Scientific |
| pPiacH-gfp[tagless] | PiacH fragment (F-R) fused to gfp[tagless] | This study |
| pPiacH-gfp[LVA] | PiacH fragment (F-R) fused to gfp[LVA] | This study |
| pPiacH-gfp[AAV] | PiacH fragment (F-R) fused to gfp[AAV] | This study |
| pPiacH-gfp[ASV] | PiacH fragment (F-R) fused to gfp[ASV] | This study |
| piacR-PiacH-gfp[AAV] | iacR-PiacH fragment fused to gfp[AAV] | This study |
| pPiacH-gfp[AAV]FNDR1 | PiacH subfragment (F1-R) fused to gfp[AAV] | This study |
| pPiacH-gfp[AAV]FNDR2 | PiacH subfragment (F2-R) fused to gfp[AAV] | This study |
| pPiacH-gfp[AAV]FNDR3 | PiacH subfragment (F3-R) fused to gfp[AAV] | This study |
| pPiacH-gfp[AAV]FNDR3A | PiacH subfragment (F3A-R) fused to gfp[AAV] | This study |
| pPiacH-gfp[AAV]FNDR3B4 | PiacH subfragment (F3B4-R) fused to gfp[AAV] | This study |
| pPiacH-gfp[AAV]FNDR4 | PiacH subfragment (F4-R) fused to gfp[AAV] | This study |
| pPiacH-gfp[AAV]FNDR5 | PiacH subfragment (F5-R) fused to gfp[AAV] | This study |
| pPiacH-gfp[AAV]FNDR1REV | PiacH subfragment (F-R1) fused to gfp[AAV] | This study |
| pPiacH-gfp[AAV]FNDR2REV | PiacH subfragment (F-R2) fused to gfp[AAV] | This study |
| pPiacH-gfp[AAV]FNDR3REV | PiacH subfragment (F-R3) fused to gfp[AAV] | This study |
| pPiacH-gfp[AAV]FNDR3AREV | PiacH subfragment (F-R3A) fused to gfp[AAV] | This study |
| pPiacH-gfp[AAV]FNDR3BREV | PiacH subfragment (F-R3B) fused to gfp[AAV] | This study |
| pPiacH-gfp[AAV]FNDR4REV | PiacH subfragment (F-R4) fused to gfp[AAV] | This study |
| pPiacH-gfp[AAV]FNDR5REV | PiacH subfragment (F-R5) fused to gfp[AAV] | This study |