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Effects of pH and temperature on TpGUS79A activity. (a) Optimum temperature; (b) optimum pH value; (c) thermostability; (d) pH stability. ■, glycine-HCl buffer (pH 2.0 to 3.0); ●, acetate buffer (pH 3.0 to 5.5); ▲, phosphate buffer (pH 5.5 to 7.5); ○, Tris-HCl buffer (pH 7.5 to 8.5); □, glycine-NaOH (pH 8.5 to 10). The experiments were performed in triplicate, and the error bars show 1 standard deviation.
