TABLE 1.
Purification step | Total protein (mg) | Total activity (U) | Sp act (U mg−1) | Fold purification | Yield (%) |
---|---|---|---|---|---|
Culture supernatant | 310.50 | 15.19 | 0.048 | 1.00 | 100 |
50% acetone precipitation | 36.63 | 11.43 | 0.31 | 6.46 | 75.25 |
HiTrap SP FF chromatography | 0.23 | 2.10 | 9.40 | 195.83 | 13.82 |
HiTrap DEAE HP chromatography | 0.06 | 0.712 | 11.97 | 249.38 | 4.69 |