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. 2018 Sep 17;84(19):e00755-18. doi: 10.1128/AEM.00755-18

TABLE 1.

Purification of the enzyme converting GL into GAMG from T. pinophilus Li-93

Purification step Total protein (mg) Total activity (U) Sp act (U mg−1) Fold purification Yield (%)
Culture supernatant 310.50 15.19 0.048 1.00 100
50% acetone precipitation 36.63 11.43 0.31 6.46 75.25
HiTrap SP FF chromatography 0.23 2.10 9.40 195.83 13.82
HiTrap DEAE HP chromatography 0.06 0.712 11.97 249.38 4.69