TABLE 2.
Substrate specificity of TpGUS79A
| Substrate | Relative activity (%)a | Km (mM) | kcat (s−1) | kcat/Km (mM−1 s−1) |
|---|---|---|---|---|
| GL | 100 | 0.54 ± 0.04 | 6.0 ± 0.4 | 11.14 ± 1.0 |
| GAMG | NDb | ND | ND | ND |
| GAM-Glc | ND | ND | ND | ND |
| Quercetin 3-glucuronide | 51.2 | 0.76 ± 0.04 | 2.9 ± 0.3 | 3.83 ± 0.5 |
| Quercetin 3-glucoside | ND | ND | ND | ND |
| Baicalin | 13.2 | 6.88 ± 0.55 | 2.0 ± 0.2 | 0.29 ± 0.01 |
| Baicalein-7-glucoside | ND | ND | ND | ND |
| pNP-β-GlcA | 5.8 | 25.56 ± 1.84 | 0.26 ± 0.01 | 0.01 ± 0.001 |
| pNP-β-Glc | ND | ND | ND | ND |
| pNP-β-Gal | ND | ND | ND | ND |
| pNP-β-Xyl | ND | ND | ND | ND |
| pNP-β-M | ND | ND | ND | ND |
a
Assays were carried out in 50 mM acetate buffer (pH 4.5) at 45°C. The activity on GL was taken as 100%.
b
ND, could not be detected.