TABLE 1.

Comparison of diversity and novelty of microorganisms isolated from soil and sediment samples by using different isolation procedures

Microorganism	No. of isolates	No. of OTUsa	Shannon diversity index	No. of novel OTUsb	Novelty indexc
Slow-growing isolates
Soil PT	29	16	2.49	1	0.034
Soil PS	28	18	2.77	8	0.286
Sediment PT	45	13	1.75	2	0.044
Sediment PS	70	34	3.02	16	0.229
Fast-growing isolatesd
Soil PT	169	27	2.48	2	0.012
Soil PS	355	52	2.97	6	0.017
Sediment PT	303	44	2.79	6	0.020
Sediment PS	503	69	3.30	22	0.044

^aIsolates were assigned to OTUs with a cutoff value of 97% identity on the basis of their partial 16S rRNA gene sequences.

^bOTUs with less than 80% classification reliability at the genus level in RDP Classifier analysis were defined as novel OTUs.

^cNovelty index was calculated by the number of novel OTUs/total number of isolates.

^dData on fast-growing isolates were from our previous work (20).