FIG 8.

Effects of sorbitol on chorismate mutase function in vivo. Panel A shows the growth of the deletion and complemented cells in medium supplemented with tyrosine and phenylalanine. Panel B depicts the growth pattern in minimal medium plus 200 ng/ml tetracycline. Cells in supplemented medium (A) can grow until osmolalities between 1.35 and 1.82 are reached, while cells in minimal medium (B) can grow confluently until osmolalities between 0.65 and 1.04 are reached. Table S1 lists the osmolalities at which cells stop growing.