TABLE 3.
Strains and plasmids used in this study
| Strain or plasmid | Source | Genotype or description |
|---|---|---|
| Strains | ||
| DH5α | Invitrogen | F− ϕ80lacZΔM15(lacZYA-argF) U169 recA1 endA1 hsdR17 (rK− mK+) phoA supE44 λ− thi-1 gyrA96 relA1 |
| NM522 | Howella | supE thi Δ(lac-proAB) Δ(mcrB-hsdS) (r– m–) [F′ proAB lacIqZΔM15] |
| NM522 thyA | Howella | supE thi Δ(lac-proAB) hsdS (r− m−) [F′ proAB lacIqZΔM15] thyA |
| LH18 | Howella | NM522 thyA ΔfolA::kan |
| BW25113 | CGSC | F− Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ− rph-1 Δ(rhaD-rhaB)568 hsdR514 |
| JW2535-1 | CGSC | BW25113 ΔglyA725::kan |
| JW3913-1 | CGSC | BW25113 ΔmetF728::kan |
| KA12/pKIMP-UAUC | Colquhounb | Δ(srlR-recA)306::Tn10 Δ(pheA-tyrA-aroF) thi-1 endA-1 hsdRl7 Δ(argF-lac) U169 supE44. The strain is complemented by the pKIMP plasmid (=pACYC184, which confers chloramphenicol resistance and carries the pheC and tyrA genes), which restores Phe and Tyr biosynthetic pathways. |
| Plasmids | ||
| CM-pKTS | Kast and Hilvertc | Ampicillin resistance, Ptet promoter control of CM expression, SsrA tag added |
| R67 DHFR-pKTS | This studyd | Ampicillin resistance, Ptet promoter control of R67 DHFR expression, SsrA tag added, trimethoprim resistance when protein expressed |
| Quad4-pKTS | This studyd | Ampicillin resistance, Ptet promoter control of Quad4 expression, SsrA tag added, trimethoprim resistance when protein expressed |
| MTHFR-pKTS | This studyd | Ampicillin resistance, Ptet promoter control of MTHFR expression, SsrA tag added |
| SHMT-pKTS | This studyd | Ampicillin resistance, Ptet promoter control of SHMT expression, SsrA tag added |