Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
List of PCR primer sequences used to introduce NdeI and XhoI restriction enzyme sitesa
| Enzyme (plasmid) | Primer sequence |
|---|---|
| MTHFR (pCAS30) | 5′ TATTTACATATGAGCTTTTTTCACGCCAGC 3′ (NdeI) |
| 5′ AAGGGGTTATGCTAGTTATTGCTCA 3′ (reverse primer) | |
| SHMT (pBSGlyA) | 5′ GGGAGGAGGCATATGTTAAAGCGTGAAATGAACATTGCCGATTATGATGCC 3′ (NdeI) |
| 5′ GAGAGAGAGCTCGAGTGCGTAAACCGGGTAACGTGC 3′ (XhoI) (reverse primer) |
Introduced restriction enzyme sequences are underlined.
