FIG 3.

Induction kinetics and dose response for the l-arabinose-, l-rhamnose-, acrylate-, and cumate-inducible systems. (A) Normalized relative fluorescence of C. necator H16 harboring the l-arabinose-, l-rhamnose-, acrylate-, and cumate-inducible systems. Inducers were added at time zero and fluorescence was monitored for 14 h. The darker the color shade, the higher the inducer concentration. l-Arabinose was supplemented to final concentrations of 10, 2.5, 1.25, 0.625, and 0.313 mM or omitted. l-Rhamnose was supplemented to final concentrations of 10, 5, 2.5, 1.25, and 0.625 mM or omitted. Acrylate was supplemented to final concentrations of 5, 1, 0.5, 0.25, and 0.05 mM or omitted. Cumate was supplemented to final concentrations of 12.5, 3.13, 1.56, 0.78, and 0.16 μM or omitted. The standard deviations from three biological replicates are illustrated as lighter shading above and below the induction kinetics curve. (B) Dose response of C. necator H16 harboring the l-arabinose-, l-rhamnose-, acrylate-, and cumate-inducible systems four (square) and eight (circle) hours after inducer addition. Error bars represent SDs from three biological replicates.