TABLE 2.
Primers used in this study
| Primer | Sequence (5′–3′) | Purpose |
|---|---|---|
| 27F | AGAGTTTGATCCTGGCTCAG | To amplify the 16S ribosomal RNA gene |
| 1492R | TACGGCTACCTTGTTACGACTT | |
| IH-F | TAAGAAGGAGATATACATATGTCAGATCAGTTGTGGTCAAAGAGTG | Construction of plasmid pET-ipaH |
| IH-R | GTGGTGGTGGTGGTGCTCGAGACCAGCGTTGATGAACGGC | |
| KA-F | TATGACCATGATTACGAATTCTTGGGGGAAGGCGCCATA | Construction of plasmid pEX-ipaH |
| KA-R | CAGGTCGACTCTAGAGGATCCTCAGTAGCCAGCCGCGGC | |
| KT-F | TCCGACCCAGCGGGAGACC | Detect integrated sequence in strain YJN-5M |
| KT-R | GCCGTGCGAGTCAGATGGA | |
| CP-F | GATAAGCTTGATATCGAATTCCGCGATGAGAAAGCAGAAATG | Construction of plasmid pBBR1-ipaH |
| CP-R | CGCTCTAGAACTAGTGGATCCCTAACCAGCGTTGATGAACGG | |
| ipaH-A | ATGTCAGATCAGTTGTGGTCAAAGAGTGCTA | Amplify ipaH gene |
| ipaH-B | CTAACCAGCGTTGATGAACGGCGAGA | |
| K82A-F | CCCGATCACCCTCGCGGTGAATATTGACCTCGTCGGT | Amplify mutant gene ipaH-K82A with Lys82 replaced Ala82 |
| K82A-R | CAATATTCACCGCGAGGGTGATCGGGACGC | |
| S157A-F | GCTGGCGGAGCGTCAGGCGGCGAG | Amplify mutant gene ipaH-S157A with Ser157 replaced to Ala157 |
| S157A-R | GCCTGACGCTCCGCCAGCCGTGC | |
| S181A-F | GATCTCGTTGGTGCGCTGCGGAATCCTGCG | Amplify mutant gene ipaH-S181A with Ser181 replaced to Ala181 |
| S181A-R | GATTCCGCAGCGCACCAACGAGATCATTCCCGAC |