Figure 8.

Lower Ploidy in AhR−/− Liver Could Be Associated with Altered Centrosome Composition

(A) Total protein was obtained from preweaning and adult AhR+/+ and AhR−/− liver and analyzed for pericentrin (PCN) expression by immunoblotting using a specific antibody.

(B) Livers from adult AhR+/+ and AhR−/− mice were fixed, embedded in paraffin, sectioned, and analyzed for PCN expression by immunofluorescence using a specific primary antibody and an Alexa 633-labeled secondary antibody. Sections were visualized using an Olympus FV1000 confocal microscope (Olympus). DAPI was used to label cell nuclei. Details of centrosomes at higher magnification are shown within yellow boxes on the right panels.

(C and D) Centrosomal area (C) and PCN protein amount (D) were quantified using the ImageJ software, and the data show the different ranges in which centrosomes can be clustered for each mice genotype. β-Actin was used to confirm protein integrity and equal loading.

Two representative mice for each condition are shown. Six mice for each developmental time and genotype and four technical replicates were analyzed. Data are shown as mean ± SD. Scale bar, 15 μm. Antibodies used are indicated in Table S1. SD, standard deviation.