Figure 5.
Expression Pattern of Viral Mimicry Genes in PD-L1IND and PD-L1CON Cell Lines and Patients with Melanoma
(A) Mean-centered heatmap of the expression level (log2 FPKMs) of 30 viral mimicry and immune-system-related genes in PD-L1IND and PD-L1CON cell lines.
(B) Heatmap of the expression level (scaled Z score) of the same set of 30 genes in TCGA skin cutaneous melanoma data stratified by TIL−/PD-L1− (group 1, representative of inducible in patient group) or TIL−/PD-L1+ (group 2, representative of constitutive in patient group). In both (A) and (B), significantly differentially expressed genes are indicated with an asterisk (*) and the gene names in a box are DNMTi-responsive genes (i.e., previously shown to be silenced and re-expressed upon DNMTi treatment in cancer).
(C–G) Gene expression of five of the nine selected ERV genes as measured by RT-qPCR. There is a higher expression of five ERV genes (MLTA10, MER21C, MLT1C627, MER4D, MER57B1) in the PD-L1CON cell lines compared with the PD-L1IND group. Error bars represent SE of two technical replicates.