Figure 1.

Pre-malignant Immortalized Cells Have a Migratory Phenotype, whereas Ras-transformed Cells Have Increased Invasive Capacity

(A) Genetically defined system of breast cancer progression.

(B) Wound-healing scratch assay: Shown are representative photographs and quantification of scratch closure after 24 hr for four biological replicates. Data are represented as mean ± SEM. SEM, standard error of the mean.

(C) Transwell assay: Shown are representative images and the mean and standard deviation of three biological replicates, quantified as percentage that migrated through Matrigel versus through the control membrane without Matrigel. Data are represented as mean ± SEM.

See also Figure S1