Figure 4.

Knockdown of α-Adducin Enhances Invasion of Immortalized Cells

(A) Western blot validation of the LC-MS/MS detected decrease of ADD1 in TFO versus IMO cells and shRNA knockdown of ADD1 in IMO cells.

(B) qRT-PCR of ADD1-encoding mRNA in IMO and TFO cells, shown as relative expression to PRIM cells, normalized to Glyceraldehyde 3-phosphate dehydrogenase (GapDH). Data are represented as mean ± standard deviation, n = 4.

(C) Transwell assay: Shown are representative images and the mean and standard deviation of two biological replicates of each shRNA, six biological replicates of IMO, and four biological replicates of TFO, quantified as percentage that migrated through Matrigel versus through the membrane without Matrigel. Data are represented as the mean ± SEM.