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. 2018 Sep 19;3(5):e00393-18. doi: 10.1128/mSphere.00393-18

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Copyright © 2018 Foe et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — Δash4 parasites form multiple residual bodies per vacuole. (A) Still frames from time-lapse microscopy of dividing wild-type and Δash4 parasites. Red arrows indicate possible residual bodies. Time from the start of the movie is indicated in minutes. The scale bar is 5 μm. (B) Indirect immunofluorescence microscopy of ROP1 localization in wild-type and Δash4 parasites. Anti-SAG2A signal is shown as a marker for parasites, anti-ROP1 highlights the apical end of the parasites, and DAPI shows nuclei. The scale bar is 5 μm. (C) Indirect immunofluorescence microscopy of MyoI-HA in untagged wild-type (WT), MYOI-HA, and Δash4 (Δash4 MYOI-HA) parasites. “αToxo” indicates parasites, anti-HA stain indicates MyoI-HA localization, and DAPI highlights nuclei. The scale bar is 5 μm.