FIG 5.

ISG20 induces a subset of antiviral genes. tet-off MEFs expressing eGFP, ISG20, or ExoII mutant were induced for 72 h, and total cellular RNA and protein were collected. (A) A selection of genes shown to be induced by ISG20 overexpression through RNA sequencing were confirmed by qRT-PCR (n = 6, 2 independent experiments). NC, no change. (B and C) IFIT1 protein induction was confirmed by Western blotting (B) and quantified (C). (A and C) *, P < 0.05; **, P < 0.01 (Mann-Whitney test). (For panels B and C, n = 2, 2 independent experiments).