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2-ME regulates eIF4E and 4E-BP1 functions in osteosarcoma cells. Cytoplasmic extracts were prepared from MG63 osteosarcoma cells (A, B) at 16 and 24 h and normal human osteoblast (HOB) cells (C, D) at 24 h following vehicle (Veh) and 2-ME treatment. The extracts were subjected to immunoprecipitation (IP) with anti-4E-BP1 antibodies (A, C) or cap-binding (B, D) and analyzed by western blot analysis using anti-eIF4E antibodies.
