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Effect of 2-ME treatment on eIF4E and 4E-BP1 expression levels. Cytoplasmic extracts were prepared following 16 and 24 h of vehicle and 2-ME treatment from MG63 cells and analyzed by western blot using anti-eIF4E, anti-4E-BP1, antiphospho-4E-BP1 (Thr37/46), anti-nonphospho-4E-BP1 (Thr46), and anti-actin antibodies.
