Fig. 3.

An efficient workflow for endothelial reprogramming and applications. Based on the fast development in the field of computational biology, an efficient workflow for endothelial reprogramming can start with using computational platform to calculate the possible sets of transcription factors to achieve efficient reprogramming. Then the protocol can be verified and optimised at the bench. Finally, ECs generated through reprogramming from patients can be used for individualised cell therapy and tissue engineering, disease modelling and drug screening.