Fig. 13.
Intracellular distribution of NGO/DOX@SPC/Cou6-FA in Hela cells by confocal microscopy. The SPC lipids were stained by coumarine-6 and the nuclei were stained by Hoechest 33342. A: blue-fluorescent of Hoechest 33342; B: red-fluorescent of DOX; C: green-fluorescent of Cou6; D: overlay of A, B, and C. The overlap yellow fluorescence revealed that SPC and NGO/DOX of NGO/DOX@SPC-FA stayed together after taken up for 1 h by Hela cells. Scale bars are 50 μm.