Figure 4.

Global RNA Sequencing Reveals Heterogeneous Gene Expression Profiles among Four EC Types (n = 3 Donors)

(A) 2D principle component analysis of RNA sequencing data for cultured organ-specific ECs showing clustered heart ECs compared with the kidney, lung, and liver ECs.

(B) Venn diagram of differentially expressed heart-specific ECs genes with respect to kidney, lung, and liver ECs.

(C and D) Gene Ontology terminology analysis (C) and volcano plot (D) showing different gene clusters for heart versus kidney ECs.

(E) Heatmaps of bona fide markers for the heart-, kidney-, and lung-specific EC transcription factors and co-factors (i), angiocrine and signaling factors (ii), and metabolism-related and other genes (iii).

(F) PCR validation of selected heart- and kidney-specific genes for additional three donor sets.