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. 2018 Sep 20;13(9):e0203657. doi: 10.1371/journal.pone.0203657

Fig 3. IL10 gene expressions on PBMC of RP patients.

Fig 3

(A) IL10 gene expressions of RP PBMC compared with those of normal PBMC. We measured IL10 gene expressions of freshly isolated, 6-hour-cultured, and 24-hour-cultured PBMC in RP patients (RP) and normal individuals (NI). Gene expressions of RP patients were estimated by the ΔΔCt method, followed by normalization of the titers relative to 2-ΔΔCt of normal individuals. The gene expression levels of normal individuals were defined as 1.000. IL10 gene expressions of freshly isolated PBMC were significantly higher in RP patients than those in normal individuals. IL10 gene expressions were significantly lower in RP patients than those of normal individuals 6 hours after the initiation of the cell culture, regardless of the presence and absence of mitogen stimulation. (B) Time-dependent changes of IL10 gene expressions of RP PBMC. We compared the gene expression data of the freshly isolated PBMC with those of cultured PBMC in patients with RP. IL10 gene expressions decreased significantly regardless of the presence and absence of mitogen stimulation in RP patients after the initiation of the cell culture. Relative gene expressions of RP patients against those of normal individuals were displayed with dot plots. A box-plot and a mean level (green line) of each group of RP patients and normal individuals were indicated.