Table 7. Primers used for RT-PCR (1μl each containing/presenting 10pmol).
Metabion international AG, Martinsried, Germany.
| Protein | Manufacturer | Annealing Temperature | Sequences 5´-3´ | Bands |
|---|---|---|---|---|
|
SP-A (A2A4) |
Metabion | 56.5°C |
F:GATGGGCAGTGGAATGACAGG R:GGGAATGAAGTGGCTAAGGGTG |
212bp |
|
SP-B (Mature Strict) |
Metabion | 60°C |
F: CACCATGTTCCCCATTCCTCT R:TCATCCATGGAGCACCGGAGGACGA |
239bp |
|
SP-C Real 1 |
Metabion | 55.8°C |
F:TCATCGTCGTGGTGATGGTG R:ATGGAGAAGGTGGCAGTGGTAA |
110bp |
|
SP-D Real 1 |
Metabion | 60°C |
F:TGCTGCTCTTCCTCCTCTCTGC R:GGGCGTTGTTCTGTGGGAGTAG |
95bp |
| GAP-DH | Metabion | 60°C |
F: CCCTTCATTGACCTCAACTAC R: CCACCTTCTTGATGTCATCAT |
300bp |