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. 2018 Sep 20;13(9):e0203502. doi: 10.1371/journal.pone.0203502

Table 8. Primers used for qRT-PCR (1μl each containing/presenting 10pmol).

Metabion international AG, Martinsried, Germany.

Protein Manufacturer Annealing Temperature Sequences 5´-3´ Bands
SP-A
Real 2
Metabion 60°C F:CATTGCTGTCCCAAGGAATC
R:CCGTCTGAGTAGCGGAAGTC
125bp
SP-B
Real 3 Roche
Metabion 60°C F:GGGTGTGTGGGACCATGT
R:CGTCTCACTTGGCTTTTCCTT
101bp
SP-C
Real 2
Metabion 60°C F:ACATGAGCCAGAAACACACG
R:GGAGAAGGTGGCAGTGGTAA
109bp
SP-D
Real 1
Metabion 53°C F:TGCTGCTCTTCCTCCTCTCTGC
R:GGGCGTTGTTCTGTGGGAGTAG
125bp
18S r RNA Metabion 60°C F: GGTGCATGGCCGTTCTTA
R: TGCCAGAGTCTCGTTCGTTA
110bp