Table 8. Primers used for qRT-PCR (1μl each containing/presenting 10pmol).
Protein | Manufacturer | Annealing Temperature | Sequences 5´-3´ | Bands |
---|---|---|---|---|
SP-A Real 2 |
Metabion | 60°C |
F:CATTGCTGTCCCAAGGAATC R:CCGTCTGAGTAGCGGAAGTC |
125bp |
SP-B Real 3 Roche |
Metabion | 60°C |
F:GGGTGTGTGGGACCATGT R:CGTCTCACTTGGCTTTTCCTT |
101bp |
SP-C Real 2 |
Metabion | 60°C |
F:ACATGAGCCAGAAACACACG R:GGAGAAGGTGGCAGTGGTAA |
109bp |
SP-D Real 1 |
Metabion | 53°C |
F:TGCTGCTCTTCCTCCTCTCTGC R:GGGCGTTGTTCTGTGGGAGTAG |
125bp |
18S r RNA | Metabion | 60°C |
F: GGTGCATGGCCGTTCTTA R: TGCCAGAGTCTCGTTCGTTA |
110bp |