Fig 5. Differential expression of Abscisic acid insensitive 1b (ABI1-B), ddb1- and cul4-associated factor (DDB1-CUl4), pathogenesis-related homeodomain (PRH), Pseudomonas syringae pv. tomato R-gene (Pto)-interactor 1 (Pti1), resistance gene analogue 1 (RGA1) and resistance gene analogues 71 (RGA71) among lentil genotypes infected with Ascochyta lentis.

A. Expression data (means and standard deviations) measured by quantitative real-time PCR (qRT-PCR) based on three biological replicates. Cycle of threshold (C_t) values were normalized with plant β-actin gene values as a reference gene and the gene expression was reported relative to non-infected plants sampled before inoculation (mock). B. RNA-seq data were generated from samples of one of the three biological replicates used for qRT-PCR. The log₂ fold change in gene expression was calculated with Cuffdiff software by dividing fragments per kb of exon per million mapped reads (FPKM) value of infected samples to that of mock.