Table 1. Primer pairs used for assessing the expression of selected candidate defense genes by quantitative real time PCR.
Primers were designed using lentil cv. CDC Redberry sequences. ABI1-B = Abscisic acid insensitive 1b; DDB1-CUl4 = ddb1- and cul4-associated factor; PRH = pathogenesis-related homeodomain; Pti1 = Pseudomonas syringae pv. tomato R-gene (Pto)-interactor 1; RGA1 and 71 = resistance gene analogue 1 and 71. Gene IDs were generated using Cufflinks software and link data presented here to the transcript annotations in S1 File.
| Gene ID | Gene name | Sequence 5’->3’ |
|---|---|---|
| TSS6729 | ABI1-B | F: ATCCGAGGTACAATCGCAAC |
| R: CCTTGGAAACGAAACAGGAG | ||
| TSS4370 | DDB1-CUL4 | F: CTCATCCACAGGGAACAAAAA |
| R: GATTAGGTGACGAGGGCAAA | ||
| TSS7406 | PRH | F: TCATCTGAGGGCCATTCTTC |
| R: CATTCCTCCTGGAGACCAAG | ||
| TSS894 | Pti1 | F: GAGTTAAAATCGCCGTTGGA |
| R: TCCAAGAACACGGGTAGAATG | ||
| TSS25883 | RGA1 | F:AGGAAAGAACGCTTGACTGG |
| R: ACGGCTAGTAGCTGGGAATG | ||
| TSS15293 | RGA71 | F: ACCCAACGATTTTGATCAGG |
| R: ATCTCCAATGGACGGGTGTA |