Table 2. Sequence description and expression levels of candidate plant defense genes differentially expressed among lentil genotypes infected with Ascochyta lentis.
Sequence descriptions are from the BLASTx against RefSeq release 60 hits with the highest percentage of sequence identity. Gene symbols were extracted from the Arabidopsis information resource TAIR (http://www.arabidopsis.org). For genes with no gene symbol in TAIR, the abbreviation of sequence description was used. Fold change in gene expression was calculated by Cuffdiff software by dividing fragments per kb of exon per million mapped reads (FPKM) value of infected samples by that of non-infected sample collected before inoculation (mock). NA = A copy of PR-4a gene was absent in 964a-46. Hpi = hours post Ascochyta lentis inoculation. Gene IDs were generated using Cufflinks software and links data presented here to the transcript annotations in S1 File.
| Gene ID | Sequence description | Gene symbol | Genotypes | |||||
|---|---|---|---|---|---|---|---|---|
| Eston | CDC Robin | 964a-46 | ||||||
| Peak time (hpi) | Log2 fold change | Peak time (hpi) | Log2 fold change | Peak time (hpi) | Log2 fold change | |||
| TSS653 | serine/threonine-kinase Nek6-like protein | NEK6 | 12 | 1.0 | 12 | 15.0 | 36 | 1.0 |
| TSS25223 | receptor-like protein kinase 1 | RLK1 | 48 | 2.0 | 24 | 14.9 | 60 | 0.9 |
| TSS20893 | wall associated kinase-like protein | WAK | 18 | 6.0 | 6 | 15.2 | 12 | 4.5 |
| TSS894 | Pto kinase interactor 1 | Pti1 | 36 | 15.7 | 24 | 2.3 | 48 | -0.2 |
| TSS13714 | S-locus lectin kinase family protein | LECRK | 12 | 4.6 | 48 | 13.6 | 18 | -0.1 |
| TSS15246 | leu-rich receptor serine threonine protein kinase bak1 | BAK1 | 6 | 11.8 | 12 | 1.9 | 6 | 1.0 |
| TSS25469 | efr3-like protein | EFR3 | 6 | 15.2 | 36 | 1.2 | 24 | 0.8 |
| TSS15452 | cbl-interacting protein kinase | CIPK | 48 | 12.1 | 24 | 3.0 | 6 | 14.1 |
| TSS20721 | mitogen-activated protein kinase 3 (ERK1) | MAPK3 | 12 | 12.2 | 6 | -0.5 | 6 | -0.3 |
| TSS16912 | mitogen activated protein kinase 20–1 | MAPK 20–1 | 36 | 3.4 | 18 | 14.3 | 12 | 11.2 |
| TSS12352 | kinase-like protein WNK11 | WNK11 | 36 | 1.3 | 24 | 11.6 | 24 | 1.1 |
| TSS21203 | map kinase homolog ntf6-like | MAPK-ntf6 | 24 | 0.5 | 36 | 1.0 | 36 | 12.9 |
| TSS24130 | mitogen-activated protein kinase kinase | MAPKK | 12 | 2.0 | 6 | 2.3 | 18 | 12.5 |
| TSS11951 | MAPK/erk kinase 2 | MEK2 | 18 | 0.2 | 24 | 13.4 | 6 | 1.1 |
| TSS3202 | ET-responsive transcription factor 1b-like | ERF1b | 12 | 3.4 | 6 | 1.5 | 6 | 17.4 |
| TSS13281 | ET-responsive transcription factor wri1 | WRI1 | 36 | 12.6 | 18 | 14.2 | 6 | 1.7 |
| TSS20180 | ET-responsive transcription factor | ERF | 18 | 0.5 | 36 | 1.1 | 36 | 11.0 |
| TSS17746 | ethylene receptor-like | ER | 36 | 1.7 | 24 | 9.7 | 6 | 10.8 |
| TSS12237 | gaga-binding transcriptional activator | GAGA-TF | 36 | 0.3 | 12 | 0.1 | 12 | 11.3 |
| TSS6729 | abscisic insensitive 1b | ABI1-B | 24 | 0.9 | 18 | 11.2 | 36 | -0.7 |
| TSS9333 | abscisic acid-insensitive 5-like protein | ABI5 | 6 | 14.7 | 60 | 0.1 | 60 | 0.9 |
| TSS10835 | pentatricopeptide repeat-containing protein | PRP | 36 | -0.4 | 36 | 10.7 | 24 | 2.1 |
| TSS17847 | f-box protein skip16 | SKIP16 | 18 | 0.3 | 12 | 9.9 | 12 | 0.2 |
| TSS22596 | f-box fbd lrr-repeat protein at3g14710-like | FBD | 60 | 3.4 | 60 | 4.8 | 60 | 18.2 |
| TSS14333 | f-box lrr-repeat protein | FLR | 60 | 13.7 | 18 | 0.7 | 36 | 2.7 |
| TSS4370 | ddb1- and cul4-associated factor | DDB1-CUL4 | 48 | 2.5 | 24 | -0.7 | 48 | 12.0 |
| TSS4644 | ankyrin repeat domain-containing protein | AKR | 12 | -0.6 | 12 | 12.3 | 18 | -0.2 |
| TSS4558 | pathogenesis-related protein 1a | PR-1a | 18 | 6.8 | 12 | 3.4 | 36 | 11.3 |
| TSS19333 | Heat shock protein | HSP | 12 | 13.5 | 18 | 1.3 | 24 | 11.6 |
| TSS4129 | Hevein-like protein | Hel | 60 | 1.9 | 12 | 8.0 | 12 | 2.0 |
| TSS3616 | pathogenesis-related protein pr-4a- (Copy#1) | PR-4a | 12 | 2.7 | 18 | 8.6 | 24 | 9.4 |
| TSS27157 | pathogenesis-related protein pr-4a (Copy#2) | PR-4a | 12 | 2.7 | 18 | 7.8 | NA | NA |
| TSS7944 | thaumatin-like protein | TLP | 12 | 1.7 | 12 | 5.9 | 24 | 6.0 |
| TSS16562 | calcium-transporting atpase | CTA | 12 | 0.6 | 12 | 0.8 | 36 | 13.3 |
| TSS10753 | programmed-cell death protein-1 | PDCD-1 | 60 | 10.1 | 60 | -0.3 | 18 | 11.1 |
| TSS25553 | cyclin-dependent kinase g-2-like | CDK | 36 | 11.4 | 24 | 0.7 | 24 | 0.4 |
| TSS12093 | autophagy-related protein | ATG | 6 | 13.6 | 12 | 12.6 | 6 | 1.1 |
| TSS5841 | autophagy-related protein 18g-like | ATG18g | 18 | 1.9 | 6 | 14.2 | 36 | 1.8 |
| TSS18849 | cellulose synthase h1-like | CESA | 12 | 1.7 | 6 | 9.7 | 24 | 0.7 |
| TSS16022 | xyloglucan glycosyltransferase 6-like | CSLC6 | 6 | 6.4 | 18 | 14.4 | 12 | 10.4 |
| TSS25181 | callose synthase 11-like isoform x1 | CALS | 36 | 2.0 | 18 | 1.1 | 36 | 11.4 |
| TSS7406 | pathogenesis-related homeodomain | PRH | 12 | 0.7 | 24 | 1.9 | 36 | 16.2 |
| TSS948 | tga transcription factor | TGA | 6 | 7.9 | 12 | 1.9 | 48 | -0.3 |
| TSS23398 | myb-like dna-binding protein bas1 | MYB | 24 | 11.0 | 6 | 2.9 | 36 | 10.3 |
| TSS4173 | poly polymerase-like | PARP | 48 | 5.7 | 48 | 12.6 | 6 | 0.7 |
| TSS6941 | arginine amidohydrolase | ARGAH | 48 | 3.6 | 12 | 7.2 | 36 | 1.9 |