Table 1. Bacterial strains and plasmids used in this study.

Strains	Relevant characteristics	Source
E. faecalis
OG1RF	Reference sequenced strain; RifR FusR	Laboratory stock
ΔefaCBA	efaCBA deletion	This study
ΔmntH1	mntH1 deletion	This study
ΔmntH2	mntH2 deletion	This study
ΔefaCBA ΔmntH1	efaCBA deletion; mntH1 deletion	This study
ΔefaCBA ΔmntH2	efaCBA deletion; mntH2 deletion	This study
ΔmntH1 ΔmntH2	mntH1 deletion; mntH2 deletion	This study
ΔefaCBA ΔmntH1 ΔmntH2	efaCBA deletion; mntH1 deletion; mntH2 deletion	This study
OG1RF + pTG001	Empty vector, ErmR	This study
ΔefaCBA ΔmntH1 ΔmntH2 + pTG001	Triple mutant, empty vector, ErmR	This study
ΔefaCBA ΔmntH1 ΔmntH2 + pTG-efa	Triple mutant, efaCBA+, ErmR	This study
ΔefaCBA ΔmntH1 ΔmntH2 + pTG-mntH1	Triple mutant, mntH1+, ErmR	This study
ΔefaCBA ΔmntH1 ΔmntH2 + pTG-mntH2	Triple mutant, mntH2+, ErmR	This study
CK111	OG1Sp upp4::P23repA4, SpecR	[37]
E. coli
EC1000	Host for RepA-dependent plasmids	[68]
DH10B	Cloning host for pTG001	Laboratory stock
Plasmids
pCJK47	Donor plasmid, carries P-pheS*; ErmR	[37]
pTG001	Expression vector, ErmR	This study