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. 2018 Sep 20;13(9):e0204418. doi: 10.1371/journal.pone.0204418

Fig 1. Diagrams and representative pictures of cancer-endothelial cell adhesion measurement.

Fig 1

A single cancer cell is attached on the end of the Con A-coated tipless cantilever (A) and then brought into contact with the endothelial (HBMEC-60) cell-cell junctions (B). After a defined contact time, the cantilever is retracted (C) until the cancer cell is entirely separated from endothelial cells (D). The phase contrast image (E) shows an MB435 cell (white arrow) attached to the cantilever and in contact with a HBMEC-60 cell-cell junction. Force curves (F) are acquired during the cantilever approach (red) and retraction (blue), and the retraction curve is used to calculate adhesion forces. Scale bar in E = 10 µm.