Skip to main content
. 2018 Sep 20;13(9):e0204418. doi: 10.1371/journal.pone.0204418

Fig 3. A relationship between the total cancer-HBME adhesion and duration of cell-cell contact.

Fig 3

Adhesion strength between MB231 and MB435 to HBMEC-60 cells was measured for different contact times: 0.5, 1, 2, 5, 10, 30, 60, 120, and 300 sec. The overall total adhesion forces in MB231 (asterisks) cells at all time points were lower than those in MB435 (diamonds). In both cell lines, from 0.5 to 300 sec cell-cell contact time the total adhesion forces increased progressively 23.79-fold (from 23.27 ± 5.73 to 553.64 ± 86.10 pN) and 9.29-fold (68.81 ± 5.91 to 639.37 ± 62.50 pN) for MB231 and MB435 respectively. Note similar time courses of adhesion dynamics to HBMEC-60 displayed by both cancer cell lines, whereas the adhesion force increased dramatically within the first 10 sec (MB231) or 30 sec (MB435) of contact with HBMEC, and then more gradually As a negative control, the MB435 (triangles) and MB231(circles) cells were brought into contact with a hydrogel-coated dish instead of an endothelial monolayer. *p< 0.001 by ANOVA analysis.