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. 2018 Oct 1;9(5):785–797. doi: 10.14336/AD.2017.1028

Figure 2. TLR2/p-NF-κB/p65 expression and TLR2/p-NF-κB/p65 microglia in brain of young and aged mice stimulated with or without LPS.

Figure 2.

Mice were dropped into the bilateral nasal cavity with saline or LPS in young or aged mice for 2 months. TLR2/p-NF-κB/p65 expression in brain was assayed by Western blot. A) Representative bands of TLR2 immunostaining. B) Quantitative data of TLR2 immunostaining. TLR2/p-NF-κB/p65 positive microglia in brain was assayed by double immunohistochemistry of TLR2/p-NF-κB/p65 and CD11b. C) Representative images of TLR2 expression on CD11b+ microglia. D) Quantitative data of double positive cells. E) Representative bands of p-NF-κB/p65 immunostaining. F) Quantitative data of p-NF-κB/p65 immunostaining. G) Representative images of p-NF-κB/p65 expression on CD11b+ microglia. H) Quantitative data of double positive cells. Quantitative results are mean ± SEM of 4 mice in each group. *p<0.05, **p<0.01.