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. 2018 Aug 24;14(8):e1007568. doi: 10.1371/journal.pgen.1007568

Fig 1. EOC generation in third instar leg discs.

Fig 1

(A) Schematic representation of the establishment of an initial PD axis and EGFR signaling events in the center of leg discs (the number of rings depicted is not meant to be accurate). (B) Schematic representation of the vn genomic locus on chromosome 3L; enhancer bashing fragments for identification of vnE represented in tan did not drive expression in leg discs, dark red drove expression in leg discs and bright red designates the minimal enhancer used for further analysis; the vnvnE-Df CRISPR deletion is represented by red bracketed bar. (C) Time-course analysis of the expression pattern of vnE-lacZ reporter gene. In young discs, expression is limited to the EOC, while in late L3 additional expression appears in medial rings. (D-F) In situ analysis of vn expression in 3rd instar leg discs with genotypes: WT (D), vnvnE-Df (E; a wing disc from the same genotype serves as a positive control). vnvnE-WT, in which Recombination mediated cassette exchange (RMCE) was used to re-introduce the wild type CRM (F). Arrowheads indicate the presence (filled) or absence (open) of EOC vn expression, arrows indicate non-EOC medial expression. (G) Schematic representation of the rho genomic locus on chromosome 3L; enhancer bashing fragments for identification of rhoE represented in tan did not drive expression in leg discs, dark red drove expression in leg discs and bright red designates the minimal enhancers used for further analysis; rhoEMIN was only used for enhancer mutagenesis; rhorhoE-Df CRISPR deletion is represented by the red bracketed bar. (H) Time-course analysis of the expression pattern of rhoE-lacZ reporter gene. In young discs, expression is limited to the EOC, while in late L3 additional expression appears in medial rings. (I-K) In situ analysis of the rho expression pattern in 3rd instar leg disc with genotype: WT (I), rhorhoE-Df (J, an eye disc from the same genotype serves as a positive control), rhorhoE-WT, in which RMCE was used to re-introduce the wild type CRM (K). Arrowheads indicate presence (filled) or absence (open) of EOC rho expression, arrows indicate medial expression and “C” indicates chordotonal organ precursor expression. Scale bar = 100μm in all figures.