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. 2018 Aug 24;14(8):e1007568. doi: 10.1371/journal.pgen.1007568

Fig 2. vnE and rhoE requirement for PD patterning of leg discs and adult legs.

Fig 2

(A-L) Effects on distal PD genes (C15 and Bar) in third instar leg discs in: (A) rhorhoE-Df vnvnE-Df /+; (B) rhorhoE-Df vnvnE-Df; (C) rhorhoE-Df vnvnE-D.vir; (D) grkΔFRT; Krn27-7-B; (E) grkΔFRT; rhorhoE-Df vnvnE-Df Krn27-7-B; (F,G) ruinga rhorhoE-Df vnL6 mutant clones; (H,I) ru1 rho7M43 vnvnE-Df mutant clones; (J) spi vn double RNAi; (K) Egfrtsla/+ WT leg disc at restrictive temperature; and (L) Egfrtsla mutant leg discs at restrictive temperature. (M-R) Adult leg morphology of: (M) WT; (N) rhorhoE-Df vnvnE-Df mutant; (O) spi vn double RNAi driven by Dll-Gal4 (legs with tarsal segments I-II: n = 21/102, I-III: n = 19/102, I-IV: n = 35/102, I-V: n = 27/102); (P) Egfrtsla mutant at restrictive temperature (remaining 10/42 legs less severely truncated); (Q) rhorhoE-Df vnvnE-D.vir; and (R) grkΔFRT; Krn27-7-B mutant. n refers to number of individual legs with a given number of tarsal segments present. Arrowheads indicate intact (filled) or perturbed (open) tarsal segments.