Table 1. Primers for the 10 reference candidate genes for the qPCR analysis in the inguinal ring of pigs.
| Gene | Function | Primer Sequences (5’– 3’) | Ensembl ID |
|---|---|---|---|
|
HMBS hydroxymethylbilane synthase |
Third enzyme of the biosynthetic pathway of the Heme group |
F: AGGATGGGCAACTCTACCTGA R: ATGGATGGTGGCCTGCATAG |
ENSSSCG00000015108 |
|
RPL19 ribosomal protein L19 |
Ribosomal protein 60S subunit component, L19E family |
F: ACCGCCACATGTATCACAGTC R: TGTGCTCCATGAGAATCCGC |
ENSSSCG00000017509 |
|
RPL32 ribosomal protein 32 |
Ribosomal protein 60S subunit component, L32E family |
F: CAAAATTAAGCGGAACTGGCGG R: GCACATTAGCAGCACTTCAAGC |
ENSSSCG00000027637 |
|
EEF1A1 eukaryotic translation elongation factor 1 alpha 1 |
Enzymatic delivery of aminoacyl tRNAs to the ribosome. |
F: CCGCCAGGACACAGGT R: TTCCCATCTCCGCAGCCT |
ENSSSCG00000004489 |
|
H3F3A H3 histone, family 3A |
3rd component of nuclear histones |
F: CTTTGCAGGAGGCAAGTGAG R: TGGCATGGATAGCACACAGG |
ENSSSCG00000023971 |
|
RPL13A ribosomal protein 13A |
Ribosomal protein 60S subunit component, L13A family |
F: CCAAGCAGGTACTTCTGGGC R: GGCAGCATGCCTCGCA |
ENSSSCG00000003166 ENSSSCG00000003167 |
|
TOP2B topoisomerase (DNA) II beta |
DNA transcription and replication |
F: AGAAGAGCTGCTGCTGAAAGG R: TCCCCGTCATTTGTCACAGG |
ENSSSCG00000011213 |
|
SDHA succinate dehydrogenase complex flavoprotein subunit A |
Encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, in the mitochondrial respiratory chain |
F: TTGTACGGAAGGTCTCTGCG R: GATGACTCCACGACACTCCC |
ENSSSCG00000020686 |
|
YWHAZ tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta |
Regulation of signal transduction pathways through binding phosphoserine proteins |
F: ATCAGATTGGGTCTGGCCCT R: GGTATCCGATGTCCACAATGTC |
ENSSSCG00000006062 |
|
PPIA peptidyl-prolyl cis-trans isomerase A |
Accelerate the folding of proteins |
F: GCGTCTCCTTCGAGCTGTTT R: ACTTGCCACCAGTGCCATTA |
ENSSSCG00000016737 |
F: forward; R: reverse.