Table 2. Average Ct means for the 10 reference candidate genes by group in each experiment.

Ct Mean ± SD
	Experiment 1			Experiment 2
Gene	Normal	Affected	Average	Normal	Affected	Average
HMBS	21.64 ± 0.39	21.82 ± 0.86	21.73 ± 0.66	21.89 ± 0.57	22.01 ± 0.25	21.95 ± 0.41
RPL19	15.12 ± 0.50	15.15 ± 0.80	15.14 ± 0.65	13.75 ± 0.53	13.51 ± 0.27	13.63 ± 0.41
RPL13A	12.56 ± 0.47	13.45 ± 2.14	13.00 ± 1.57	14.02 ± 0.53	14.07 ± 0.95	14.05 ± 0.71
TOP2B	18.15 ± 0.81	18.11 ± 1.95	18.13 ±1.45	18.85 ± 0.54	18.93 ± 0.43	18.89 ± 0.45
RPL32	14.05 ± 0.47	14.12 ± 1.31	14.09 ± 0.96	13.57 ± 0.76	13.20 ± 0.57	13.39 ± 0.65
SDHA	19.93 ± 1.32	20.80 ± 1.90	20.37 ± 1.45	20.55 ± 1.16	21.13 ± 1.43	20.84 ± 1.24
EEF1A1	10.86 ± 0.88	10.89 ± 2.62	10.88 ± 1.89	12.34 ± 0.75	12.90 ± 1.39	12.62 ± 1.08
H3F3A	15.90 ± 0.65	16.34 ± 0.83	16.12 ± 0.76	18.35 ± 0.91	19.15 ± 1.72	18.75 ± 1.34
YWHAZ	19.90 ± 2.09	19.92 ± 2.65	19.91 ± 2.32	15.85 ± 0.43	15.30 ± 0.36	15.58 ± 0.47
PPIA	-	-	-	15.77 ± 0.33	16.18 ± 0.23	15.98 ± 0.34