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. 2018 Sep 20;9:3835. doi: 10.1038/s41467-018-06148-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Changes in the integrity of 16HBE^rs35699176 epithelial monolayers influence A. fumigatus infection. Blue: 16HBE; Red: 16HBE^rs35699176. a A. fumigatus conidia shows greater adhesion to cells and extracellular matrix components of 16HBE^rs35699176 cell lines at 30 min and 2 h after infection as determined by using two-way ANOVA with Sidak multiple comparison tests. b Extracellular matrix protein concentration is higher in 16HBE^rs35699176 than 16HBE cells. Differences between protein concentrations were performed using an unpaired T-test after performing normality test using D’Agostinho and Pearson normality test. c Germination time for A. fumigatus conidia inoculated onto 16HBE^rs35699176 (red) monolayers compared to 16HBE (blue) is decreased in the variant cell line. Differences were analysed using two-way ANOVA with multiple comparison test. d A. fumigatus CEA10 hyphal extension is increased in 16HBE^rs35699176 compared to 16HBE cells. Differences were analysed using an unpaired T-test after normal distribution being confirmed using the D’Agostinho and Pearson normality test. e Lactate dehydrogenase (LDH) assays show increased A. fumigatus CEA10 cytotoxicity in 16HBE^rs35699176 after 20 h infection. Differences were analysed using a non-parametric Mann–Witney test post-D’Agostinho and Pearson normality test. f Germination time of A. fumigatus spores in conditioned culture supernatant is reduced in 16HBE^rs35699176 cell lines (red) at 6 h infection time. Differences were analysed using a two-way ANOVA with multiple comparison test. g Significantly reduced spore germination of A. nidulans, A. flavus and A. terreus in 16HBE and 16HBE^rs35699176 cell supernatants compared to A. fumigatus (Supplementary Figure 2). Differences were analysed using one-way ANOVA with Dunnet’s multiple comparison test. h A. fumigatus conidia are more effectively internalized by cells carrying the variant rs35699176 than in 16HBE controls at 2 h co-culture. Differences were analysed using two-way ANOVA with multiple comparison test. i Increased IL-10 and IL-6 release in 16HBE^rs356999176 cells in response to A. fumigatus exposure for 6 h. Transparent fill denotes A. fumigatus exposure. Differences were analysed using two-way ANOVA with multiple comparison test. Data in graphs are represented as mean ± standard deviation. Means were compared; *P < 0.05. **P < 0.001, ***P < 0.0001