Figure 4.

Chaperoning activity of reduced EcAhpC. (A) SEC elution profile of 10 µM of reduced EcAhpC treated at 25 °C (black) and 53 °C (blue) for 1 h. A very large shift in the elution volume is observed for sample treated at 53 °C (blue), demonstrating the formation of HMW oligomers. The trace indicated on the elution peak was collected (F1 to F6) for SDS-PAGE analysis. (B) DLS data present the increase in diameter of reduced EcAhpC after heating of the enzyme at 48 °C for 10 min (−). (C) 1 µM LDH aggregation (black) was greatly reduced in the presence of reduced EcAhpC at 48 °C (red).