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. 2018 Sep 20;8:14143. doi: 10.1038/s41598-018-32495-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell adhesion and distribution in the microgrooved DEX-BCP-Col composite scaffolds. (a) SEM images of the scaffolds after 1 day of in vitro culture. Cells in the top layers and basal layers are shown at low and high magnifications. (b) Distribution of HUVECs after 3 days of in vitro culture. Blue fluorescence: cell nuclei stained by DAPI. (c) Assembly of HUVECs in the microgrooves after 3 days of in vitro culture. Red fluorescence: HUVECs immunologically stained with human-CD 31. Blue fluorescence: cell nuclei stained by DAPI.