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. 2018 Sep 20;8:14143. doi: 10.1038/s41598-018-32495-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Staining and quantification of the newly formed blood vessels in the implants after 8 weeks of implantation. (a) Photomicrographs of H&E staining of the decalcified cross-sections of the implants at a high magnification of the peripheral and central regions. Yellow triangles indicate new formed blood vessels. (b) Immunohistochemical staining of vWF in the peripheral and central regions of the implants. The brown signals show the presence of vWF. (c) The microvessel density (MVD) within the implants. MVD was quantified by using the cross-sections immunohistochemically stained for vWF. Data represent means ± SD, n = 4. Significant difference: *p < 0.05; **p < 0.01; ***p < 0.001.