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. 2018 Sep 20;9(10):959. doi: 10.1038/s41419-018-0999-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a After surgery, all rats were exposed to X-ray to affirm the model was successful. The images were collected at 1, 4 and 8 weeks after implantation. b, d Micro-CT 3D reconstruction images of the new bone formation and β-TCP matrix at 1, 4 and 8 weeks after surgery. c BV/TV was used to evaluate new bone formation. e RSV/SV was used to evaluate scaffold degradation. n = 10 samples per group. Data are represented as the mean ± s.e.m. *P < 0.05, **P < 0.01 as determined by Student’s t-tests. f H&E staining of rat femur sections at 4 and 8 weeks after the surgery