Fig. 8. RAOECs regulate BMSC differentiation into endothelial cells via the FGF2-RhoA/ROCK signaling pathway.

a Level of FGF2 in the control group and the co-culture group, n = 3. Data are represented as the mean ± s.e.m. *P < 0.05; **P < 0.01; ***P < 0.001as determined by Student’s t-tests. b Relative FGF2 mRNA expression in the control group and the co-culture group, n = 3. Data are represented as the mean ± s.e.m. **P < 0.01; ***P < 0.001 as determined by Student’s t-tests. c, e qRT-PCR analysis of RhoA, ROCK-1, ROCK-2 and CD31 in BMSCs from groups with different treatments, n = 3. Data are represented as the mean ± s.e.m. *P < 0.05; **P < 0.01; ***P < 0.001 as determined by Student’s t-tests. d, f WB analysis of RhoA, ROCK-1, ROCK-2 and CD31 in BMSCs from groups with different treatments. g FGF2, secreted by endothelial cells, binds with FGFRs on BMSC membranes and promotes GDP-RhoA translation into inactive p-RhoA but not active GTP-RhoA