Table 1.
Information of lines used in the different experiments hLFs
| Control Lines |
IPF Lines |
||||
|---|---|---|---|---|---|
| Experiment | Size, n | Average age, yr (SD) | Size, n | Average age, yr (SD) | P Value Age |
| Actin stain | 3 | 68 (5) | 4 | 65 (7) | 0.63 |
| Proliferation assay | 11 | 64 (6) | 8 | 63 (8) | 0.86 |
| Telomere length | 5 | 64 (4) | 5 | 66 (6) | 0.61 |
| β-Galactosidase activity | 4 | 66 (5) | 4 | 68 (4) | 0.55 |
| mRNA expression senescence (nonstimulated) | 5 | 64 (4) | 9 | 61 (8) | 0.36 |
| Protein expression senescence markers | 4 | 66 (5) | 3 | 62 (6) | 0.41 |
| mRNA expression senescence markers, collagen 1, α-SMA, BiP, and XBP1 (TGF-β stimulated) | 4 | 66 (5) | 4 | 65 (7) | 0.96 |
| SASP mRNA expression | 3 | 65 (6) | 3 | 69 (5) | 0.41 |
| OCAR_ECAR (nonstimulated) | 3 | 64 (5) | 3 | 62 (10) | 0.78 |
| OCAR_ECAR (TGF-β stimulated) | 3 | 67 (4) | 4 | 71 (3) | 0.23 |
| Mitotracker green | 3 | 64 (5) | 4 | 62 (8) | 0.63 |
| ATP production | 3 | 64 (4) | 3 | 71 (3) | 0.07 |
| Electron microscopy | 3 | 64 (5) | 4 | 62 (8) | 0.63 |
| Apoptosis (TUNEL assay) | 4 | 66 (5) | 4 | 68 (4) | 0.55 |
hLFs, human lung fibroblasts; IPF, idiopathic pulmonary fibrosis; α-SMA, α-smooth muscle actin; OCR, oxygen consumption rate; SASP, senescence-associated secretory phenotype; ECAR, extracellular acidification rate; TGF-β, transforming growth factor-β; TUNEL, terminal deoxynucleotidyl transferase dUTP-mediated nick-end labeling.