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. 2018 Sep 20;9(10):954. doi: 10.1038/s41419-018-0978-y

Fig. 5. Rescue experiments performed to confirm that PTPRB is the functional target of miR-665 in HCC progression.

Fig. 5

a Western blotting was conducted to examine PTPRB protein expression in the HepG2 cells transfected with miR-665 overexpression lentivirus (pre-miR-665) and PTPRB overexpression lentivirus (LV-PTPRB). b Western blotting was conducted to examine PTPRB protein expression in the SMMC-7721 cells transfected with lentivirus with short hairpin RNA targeting miR-665 (miR-665-inhibitor) and lentivirus with short hairpin RNA targeting PTPRB (PTPRB-shRNA). c, d Transwell assays of HepG2-pre-miR-665 cells transfected with LV-PTPRB or LV-NC (c), and SMMC-7721-miR-665-inhibitor cells transfected with PTPRB-shRNA or PTPRB-NC (d). e, f Soft agar growth assay of HepG2-pre-miR-665 cells transfected with LV-PTPRB or LV-NC (e), and SMMC-7721-miR-665-inhibitor cells transfected with PTPRB-shRNA or PTPRB-NC (f). g, h (EdU) incorporation analysis of HepG2-pre-miR-665 cells transfected with LV-PTPRB or LV-NC (g), and SMMC-7721-miR-665-inhibitor cells transfected with PTPRB-shRNA or PTPRB-NC (h). Three independent experiments were performed for each group. All data are represented as means ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001