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. 2018 Sep 20;8:14145. doi: 10.1038/s41598-018-32459-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a,b) 3D visualization of cartilaginous elements from a developing P. waltl limb forearm. White spots represent cell nuclei. c) Quantitative analysis allowed the determination of cell number, average size (given by ratio of volume of the whole element and number of cells), density and the volume of each cartilaginous element. The difference between diaphysis and epiphysis is evident: the density of cells in the diaphysis is half in comparison with the density in the epiphysis.