Fig. 3. No effect of miR-125b on NCCIT cells in vitro.

a CCK-8 assay of NCCIT cells at the indicated time points (24 h, 48 h, and 72 h) after transfection of miR-125b antagomir, or agomir, or their NCs. b Representative EdU incorporation staining and the percentage of EdU-positive cells were shown. c Cell cycle assay by flow cytometric analysis of PI-stained nuclei. d Apoptosis assay of transfected NCCIT cells by flow cytometric analysis of Annexin V-FITC/PI staining. e Representative TUNEL staining and quantification of fraction of TUNEL-positive cells were shown. Data were presented as the mean ± SEM (n = 3, each group). Scale bar in b and E, 25 μm