Fig. 2.

Full-length Pfs48/45 induces functional polyclonal and monoclonal antibodies in mice. a. A panel of Pfs48/45-specific mAbs was raised from splenocytes of mice immunised with full-length Pfs48/45. End point titres were determined by Pfs48/45 end point ELISA. The IgG isotypes of the individual mAbs are indicated. The mAbs were grouped by cross-competition ELISA. b Schematic depiction of competition groups in the mAb panel and 85RF45.1 and 32F3. Lines indicate cross-competition between whole groups or individual mAbs and other competition groups. c Transmission-reducing activity of the anti-Pfs48/45 mAb panel at a concentration of 375 µg/ml was determined relative to normal mouse antibody. Data points show %TRA calculated from the oocyst counts in 20 mosquito midguts for non-transmission-blocking mAbs and 40 mosquito midguts from two independent feeds for transmission-blocking mAbs, error bars show 95% CI. d Transmission-reducing activity of selected anti-Pfs48/45 mAbs was determined relative to normal mouse IgG at 350, 70 and 14 µg/ml. Data points show transmission-reducing activity calculated from the oocyst counts in 20 mosquito midguts, error bars show 95% CI