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. 2018 Sep 20;9(10):957. doi: 10.1038/s41419-018-0974-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a–d CPEB1 levels, detected by quantitative RT-PCR and immunoblotting, were significantly downregulated in data from The Cancer Genome Atlas (TCGA) and human HCC patient samples (T: tumor tissues, N: adjacent non-tumor tissues). e Immunohistochemical staining of CPEB1 in liver cancer (n = 68, odd rows) and adjacent non-cancerous liver tissues (n = 60, even rows) in a tissue microarray (TMA), the staining scores of CPEB1 in liver cancer were lower than those observed in the adjacent normal liver tissues. Immunohistochemistry staining indicated that CPEB1 immunostaining primarily occurred in the cytoplasm of liver cancer tissues cells. f The expression levels of CPEB1 by immunostaining were moderately positive, weakly positive and negative in cancer samples, stronger staining was observed in peritumoral tissues. Left image, original magnification × 10; right image, magnification × 400; the white squares indicate the area shown at higher magnification. g Correlation analysis of CPEB1 expression with CD133 expression in 80 HCC specimens by qRT-PCR analysis in biopsies and immunohistochemistry staining in paraffin-embedded tissue. The data are presented as the means ± SEM. *P < 0.05